J. Thomas August's Biological Response Mediators and Modulators. John Jacob PDF

By J. Thomas August

ISBN-10: 0120680505

ISBN-13: 9780120680504

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Dr. Christopher Peri got his Doctorate from the college of California, Berkeley, in structure. His concentration was once on Collaboration in digital Environments delving into equipment that facilitate designers and engineers to enhance verbal exchange over distant networks.

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Extra resources for Biological Response Mediators and Modulators. John Jacob Abel Symposia on Drug Development

Example text

However, the apparent rate of decay of light receptor was considerably slower in up-regulated control cells (Fig. 4, Panels A-F) compared to down-regulated cells (Fig. 4, Panels GL). Fig. 3. Insulin-binding isotherms for cell surface receptors (Panel A) and Trition X-100-extracted, isopycnically-banded receptors (Panel B). 7 x 10 6 M insulin were up-regulated for 24 h as described^ in Fig. 2. Onehalf of the cells were then down-regulated with 1 x IO"6 M insulin. An additional group was continuously maintained in medium containing insulin.

Half-replacement of light receptors by heavy receptors on the cell surface following the 3-h lag required about 7 h in cells exposed to insulin-containing medium (Fig. 9). When compared on the basis of percent of cell surface or total cellular receptors, the progress curves for surface and total heavy receptor formation and light receptor decay closely parallel each other following their respective lag periods. 5-h lag after the densityshift in the appearance of active (capable of binding insulin) total heavy cellular receptor has been observed previously (4,5).

References 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. Roberts, LJ, II, SR Marney, J r , and JA Oates (1979). N Engl J Med 300, 236. Roberts, LJ, II, RA Lewis, JA Oates and KF Austen (1979). Biochim Biophys Acta 575, 185. Lewis, RA, ST Holgate, LJ Roberts, II, JA Oates and KF Austen (1981). In Biochemistry of the Acute Allergic Reactions, pp 239254. Alan R Liss, Ine, New York. Ellis, CK, MD Smigel, JA Oates and BJ Sweetman (1979). J Biol Chem 254, 4152. Roberts, LJ, II, BJ Sweetman, RA Lewis, KF Austen and JA Oates (1980).

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Biological Response Mediators and Modulators. John Jacob Abel Symposia on Drug Development by J. Thomas August

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