By Davide Ricci, Pier Carlo Braga
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Additional info for Atomic Force Microscopy: Biomedical Methods and Applications (Methods in Molecular Biology Vol 242)
200, 109–113. 15. Barrett, R. C. and Quate, C. F. (1991) Optical scan-correction system applied to atomic force microscopy. Rev. Sci. Instrum. 62, 1393–1399. 38 Ricci and Braga Micromechanical Biosensors 39 4 Advanced Biosensing Using Micromechanical Cantilever Arrays Martin Hegner and Youri Arntz 1. Introduction During the last few years, a series of new detection methods in the field of biosensors have been developed. Biosensors are analytical devices that combine a biologically sensitive element with a physical or chemical transducer to selectively and quantitatively detect the presence of specific compounds in a given external environment.
It is useful to add that quite often (in fact, always) the sample will have a plane tilt relative to the motion of the scanner. Although all acquisition software allows for subtracting the tilt during scanning, it is good practice to try and mount the sample as planar as possible so that the piezoelectric element responsible for the vertical movement will operate across a smaller range and hence behaving linearly. 5. Thermal Drift External temperature changes or gradients will affect the AFM and its scanner depending on their mechanical properties.
03 25 Cell type Substrate Fibroblasts, etc. aA viscoelastic modulation method was adopted for the particular study. cell during actual imaging conditions, as well as providing a measure of nondestructiveness of tip–cell interactions. Optimum quality of the image can also be obtained through adjustments of fast-scan speed and direction, force loading, parameters of the feedback loop, and field of view. 2. Tip contamination: effects and diagnostics. A cell cultured in a biofluid contains proteins, cell debris, and other contaminants in solution.
Atomic Force Microscopy: Biomedical Methods and Applications (Methods in Molecular Biology Vol 242) by Davide Ricci, Pier Carlo Braga