By Margaret I. Tyler (auth.), Catherine Cooper, Nicolle Packer, Keith Williams (eds.)
Amino acid research is popular in biotechnology, biomedical, and nutrients research laboratories. Amino Acid research Protocols constitutes a huge number of those critical analytical strategies, either vintage and state of the art, of excessive software for answering particular organic questions. universal equipment comprise these in accordance with HPLC or fuel chromatography separation and research after precolumn derivatization. New recommendations in accordance with capillary electrophoresis separation, high-performance anion alternate chromatography, and mass spectrometry also are offered. due to the fact that effects rely seriously at the caliber of the pattern, such a lot individuals have dedicated a piece to pattern training, relatively to the gathering and garage of physically fluids. a brand new strategy for desalting samples ahead of hydrolysis can also be supplied. every one procedure is defined in step by step aspect to make sure profitable experimental effects, and comprises valuable notes on pitfalls to prevent, and adaptations that let the how you can be used with various systems.
updated and hugely useful, Amino Acid research Protocols deals analytical and scientific chemists, in addition to a huge variety of organic and biomedical investigators, a wealthy compendium of laboratory instruments for the effective research of either universal and unusual amino acids.
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Extra resources for Amino Acid Analysis Protocols
Chem. 272, 21932–21937. 11. Wu, G. and Furlanut, M. (1997) Separation of DL-dopa by means of micellar electrokinetic capillary chromatography after derivatization with Marfey’s reagent. Pharmacol. Res. 35, 553–556. 12. Goodnough, D. , Lutz, M. , and Wood, P. L. (1995) Separation and quantification of D - and L -phosphoserine in rat brain using N-alpha-(2, 4-dinitro-5fluorophenyl)-L-alaninamide (Marfey’s reagent) by high-performance liquid chromatography with ultraviolet detection. J. Chromatog.
And Coon, M. J. (1982) Purification and characterization of a unique isozyme of cytochrome P-450 from liver microsomes of ethanol-treated rabbits. J. Biol. Chem. 257, 8472–8480. 3. Bidlingmeyer, B. , Cohen, S. , and Tarvin, T. L. (1984) Rapid analysis of amino acids using pre-column derivatization. J. Chromatog. 336, 93–104. 4. Heinrikson, R. L. and Meredith, S. C. (1984) Amino acid analysis by reversedphase high-performance liquid chromatography: pre-column derivatization with phenylisothiocyanate.
Sample vol 5–25 mL 50–150 μL Bed vol 100 μL 500 μL ProSorb sample preparation cartridge (Applied Biosystems, Foster City, CA). , Bedford, MA). , Corning, NY) (see Note 4). Reaction Vials (Waters Associates, Milford, MA). 2. Reagents 1. 2. 3. 4. 5. HPLC-grade water (see Note 5). Trifluoroacetic acid (TFA). Methanol. Hydrochloric acid (HCl). Phenol. 3. Reagents 1. 1 N HCl. 2. 1% phenol. 3. 1% TFA. 3. 1. Preparation of the Macrospin column (sample 50–150 μ L) 1. The macrospin columns should be prepared basically as suggested by the manufacturer (2), except that we have introduced several more washes to better equilibrate the columns.
Amino Acid Analysis Protocols by Margaret I. Tyler (auth.), Catherine Cooper, Nicolle Packer, Keith Williams (eds.)